Prediction of human miRNA target genes using computationally reconstructed ancestral mammalian sequences
نویسندگان
چکیده
منابع مشابه
Prediction of human miRNA target genes using computationally reconstructed ancestral mammalian sequences
MicroRNAs (miRNA) are short single-stranded RNA molecules derived from hairpin-forming precursors that play a crucial role as post-transcriptional regulators in eukaryotes and viruses. In the past years, many microRNA target genes (MTGs) have been identified experimentally. However, because of the high costs of experimental approaches, target genes databases remain incomplete. Although several ...
متن کاملReconstructed Ancestral Sequences Improve Pathogen Identification Using Resequencing DNA Microarrays
We describe the benefit of using reconstructed ancestral sequences (RAS) on resequencing microarrays for rapid pathogen identification, with Enterobacteriaceae rpoB sequences as a model. Our results demonstrate a sharp improvement of call rate and accuracy when using RASs as compared to extant sequences. This improvement was attributed to the lower sequence divergence of RASs, which also expand...
متن کاملMutation of miRNA target sequences during human evolution.
It has long-been hypothesized that changes in non-protein-coding genes and the regulatory sequences controlling expression could undergo positive selection. Here we identify 402 putative microRNA (miRNA) target sequences that have been mutated specifically in the human lineage and show that genes containing such deletions are more highly expressed than their mouse orthologs. Our findings indica...
متن کاملDetecting compensatory covariation signals in protein evolution using reconstructed ancestral sequences.
When protein sequences divergently evolve under functional constraints, some individual amino acid replacements that reverse the charge (e.g. Lys to Asp) may be compensated by a replacement at a second position that reverses the charge in the opposite direction (e.g. Glu to Arg). When these side-chains are near in space (proximal), such double replacements might be driven by natural selection, ...
متن کاملUsing network context as a filter for miRNA target prediction
Recent discoveries in genetics show that gene expression in igher eukaryotes is regulated in part by small non-coding RNA olecules named microRNAs (miRNAs) (Ambros, 2004; Bartel, 004; Lim et al., 2003). In plants, miRNAs regulate protein xpression by binding to the coding region of the corresponding essenger RNA (mRNA). Plant miRNAs exhibit near-perfect comlementarity with the target sites. In ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2016
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/gkw1085